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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has long been noticed in lots of tumors, which results in chromosome instability, and so may well lead to tumorigenesis. This protein was discovered to affiliate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a essential part in advertising mobile-cycle progression inside the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are actually described. Two additional splice variants were being documented but detailed nucleotide sequence facts just isn't yet readily available.
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Blocking peptides are peptides that bind specifically for the concentrate on antibody and block antibody binding. These peptide generally has the epitope regarded through the antibody.
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This manual outlines further more probable optimization of mobile and immunoassay parameters to make certain the best possible results are...
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This tutorial outlines even further probable optimization of mobile and immunoassay parameters to be certain the very best success are...
Antibodies sure to the blocking peptide now not bind into the epitope over the concentrate on protein. By evaluating the staining with the blocked antibody as opposed to the antibody on your own, you can see which staining is distinct.
Goal Details Cyclin E belongs for the extremely conserved cyclin family, whose members are characterised by a spectacular periodicity in protein abundance from the mobile cycle. Cyclins function as regulators of CDK kinases. Different cyclins show unique expression and degradation patterns which add on the temporal coordination of each mitotic event. Cyclin E varieties a fancy with and functions for a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates within the G1-S period boundary and is degraded as cells development by means of S section.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by each antibodies...
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